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sssc plus  (Nextera AS)

 
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    Structured Review

    Nextera AS sssc plus
    Testing overview for <t>SSsc</t> and SSlp kits. For all testing schema, sample preparation was followed by library preparation, Illumina sequencing, and analysis using Cogent AP software. (A) Workflow for testing cultured cells (GM12878 and CHO cells) isolated by Fluorescence-activated cell sorting (FACS). This workflow was used for a performance comparison between SSsc and SS2 and for verifying compatibility with miniaturized volumes on the MANTIS (Formulatrix) and mosquito (SPT Labtech) (Figs. 2 and 5). (B) Workflow for performance comparison between SSsc and SS3. CD3+ T cells were isolated from human PBMCs by FACS, and the appropriate user manual or protocol was followed for RNA isolation (Fig. 3). (C) Workflow for performance comparison between SSsc <t>PLUS</t> (SSsc + SSlp) and Nextera XT using isolated RNA (control mouse brain RNA; Fig. 4).
    Sssc Plus, supplied by Nextera AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sssc plus/product/Nextera AS
    Average 90 stars, based on 1 article reviews
    sssc plus - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Benchmarking Single-Cell mRNA–Sequencing Technologies Uncovers Differences in Sensitivity and Reproducibility in Cell Types With Low RNA Content"

    Article Title: Benchmarking Single-Cell mRNA–Sequencing Technologies Uncovers Differences in Sensitivity and Reproducibility in Cell Types With Low RNA Content

    Journal: Journal of Biomolecular Techniques : JBT

    doi: 10.7171/3fc1f5fe.dbeabb2a

    Testing overview for SSsc and SSlp kits. For all testing schema, sample preparation was followed by library preparation, Illumina sequencing, and analysis using Cogent AP software. (A) Workflow for testing cultured cells (GM12878 and CHO cells) isolated by Fluorescence-activated cell sorting (FACS). This workflow was used for a performance comparison between SSsc and SS2 and for verifying compatibility with miniaturized volumes on the MANTIS (Formulatrix) and mosquito (SPT Labtech) (Figs. 2 and 5). (B) Workflow for performance comparison between SSsc and SS3. CD3+ T cells were isolated from human PBMCs by FACS, and the appropriate user manual or protocol was followed for RNA isolation (Fig. 3). (C) Workflow for performance comparison between SSsc PLUS (SSsc + SSlp) and Nextera XT using isolated RNA (control mouse brain RNA; Fig. 4).
    Figure Legend Snippet: Testing overview for SSsc and SSlp kits. For all testing schema, sample preparation was followed by library preparation, Illumina sequencing, and analysis using Cogent AP software. (A) Workflow for testing cultured cells (GM12878 and CHO cells) isolated by Fluorescence-activated cell sorting (FACS). This workflow was used for a performance comparison between SSsc and SS2 and for verifying compatibility with miniaturized volumes on the MANTIS (Formulatrix) and mosquito (SPT Labtech) (Figs. 2 and 5). (B) Workflow for performance comparison between SSsc and SS3. CD3+ T cells were isolated from human PBMCs by FACS, and the appropriate user manual or protocol was followed for RNA isolation (Fig. 3). (C) Workflow for performance comparison between SSsc PLUS (SSsc + SSlp) and Nextera XT using isolated RNA (control mouse brain RNA; Fig. 4).

    Techniques Used: Sample Prep, Illumina Sequencing, Software, Cell Culture, Isolation, Fluorescence, FACS, Comparison, Control

    Comparing library preparation of Nextera XT and SSsc PLUS. A) Higher library yields for SSsc PLUS (n = 6 for each; median = 10.5 and 55.4 nM, respectively). The boxes denote the interquartile range (IQR) (i.e., the 25th and 75th quartiles); the whiskers are 1.5´ IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. (B) Comparable distribution of reads for major genomic categories (i.e., mitochondria, rRNA, intergenic, intronic, and exonic; n = 6). The boxes denote the IQR (i.e., the 25th and 75th quartiles); the whiskers are 1.5´ IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. (C) Gene counts for transcripts per million (TPM) > 0.1 with SSsc PLUS compared with Nextera XT [n = 6; medians = 14,643 (SSlp) and 14,494 (Nextera XT)]. (D) Representative x-y plots of 2 example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate and the same cDNA library compared between Nextera XT and PLUS. R2 values are shown in the graph; Nextera XT R2 = 0.9961, PLUS R2 = 0.9978, and Nextera XT versus PLUS R2 = 0.9579.
    Figure Legend Snippet: Comparing library preparation of Nextera XT and SSsc PLUS. A) Higher library yields for SSsc PLUS (n = 6 for each; median = 10.5 and 55.4 nM, respectively). The boxes denote the interquartile range (IQR) (i.e., the 25th and 75th quartiles); the whiskers are 1.5´ IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. (B) Comparable distribution of reads for major genomic categories (i.e., mitochondria, rRNA, intergenic, intronic, and exonic; n = 6). The boxes denote the IQR (i.e., the 25th and 75th quartiles); the whiskers are 1.5´ IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. (C) Gene counts for transcripts per million (TPM) > 0.1 with SSsc PLUS compared with Nextera XT [n = 6; medians = 14,643 (SSlp) and 14,494 (Nextera XT)]. (D) Representative x-y plots of 2 example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate and the same cDNA library compared between Nextera XT and PLUS. R2 values are shown in the graph; Nextera XT R2 = 0.9961, PLUS R2 = 0.9978, and Nextera XT versus PLUS R2 = 0.9579.

    Techniques Used: cDNA Library Assay



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    sssc plus  (Nextera AS)
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    Nextera AS sssc plus
    Testing overview for <t>SSsc</t> and SSlp kits. For all testing schema, sample preparation was followed by library preparation, Illumina sequencing, and analysis using Cogent AP software. (A) Workflow for testing cultured cells (GM12878 and CHO cells) isolated by Fluorescence-activated cell sorting (FACS). This workflow was used for a performance comparison between SSsc and SS2 and for verifying compatibility with miniaturized volumes on the MANTIS (Formulatrix) and mosquito (SPT Labtech) (Figs. 2 and 5). (B) Workflow for performance comparison between SSsc and SS3. CD3+ T cells were isolated from human PBMCs by FACS, and the appropriate user manual or protocol was followed for RNA isolation (Fig. 3). (C) Workflow for performance comparison between SSsc <t>PLUS</t> (SSsc + SSlp) and Nextera XT using isolated RNA (control mouse brain RNA; Fig. 4).
    Sssc Plus, supplied by Nextera AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sssc plus/product/Nextera AS
    Average 90 stars, based on 1 article reviews
    sssc plus - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Testing overview for SSsc and SSlp kits. For all testing schema, sample preparation was followed by library preparation, Illumina sequencing, and analysis using Cogent AP software. (A) Workflow for testing cultured cells (GM12878 and CHO cells) isolated by Fluorescence-activated cell sorting (FACS). This workflow was used for a performance comparison between SSsc and SS2 and for verifying compatibility with miniaturized volumes on the MANTIS (Formulatrix) and mosquito (SPT Labtech) (Figs. 2 and 5). (B) Workflow for performance comparison between SSsc and SS3. CD3+ T cells were isolated from human PBMCs by FACS, and the appropriate user manual or protocol was followed for RNA isolation (Fig. 3). (C) Workflow for performance comparison between SSsc PLUS (SSsc + SSlp) and Nextera XT using isolated RNA (control mouse brain RNA; Fig. 4).

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Benchmarking Single-Cell mRNA–Sequencing Technologies Uncovers Differences in Sensitivity and Reproducibility in Cell Types With Low RNA Content

    doi: 10.7171/3fc1f5fe.dbeabb2a

    Figure Lengend Snippet: Testing overview for SSsc and SSlp kits. For all testing schema, sample preparation was followed by library preparation, Illumina sequencing, and analysis using Cogent AP software. (A) Workflow for testing cultured cells (GM12878 and CHO cells) isolated by Fluorescence-activated cell sorting (FACS). This workflow was used for a performance comparison between SSsc and SS2 and for verifying compatibility with miniaturized volumes on the MANTIS (Formulatrix) and mosquito (SPT Labtech) (Figs. 2 and 5). (B) Workflow for performance comparison between SSsc and SS3. CD3+ T cells were isolated from human PBMCs by FACS, and the appropriate user manual or protocol was followed for RNA isolation (Fig. 3). (C) Workflow for performance comparison between SSsc PLUS (SSsc + SSlp) and Nextera XT using isolated RNA (control mouse brain RNA; Fig. 4).

    Article Snippet: Outliers are plotted as empty circles. ( C ) Gene counts for transcripts per million (TPM) > 0.1 with SSsc PLUS compared with Nextera XT [ n = 6; medians = 14,643 (SSlp) and 14,494 (Nextera XT)]. ( D ) Representative x - y plots of 2 example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate and the same cDNA library compared between Nextera XT and PLUS.

    Techniques: Sample Prep, Illumina Sequencing, Software, Cell Culture, Isolation, Fluorescence, FACS, Comparison, Control

    Comparing library preparation of Nextera XT and SSsc PLUS. A) Higher library yields for SSsc PLUS (n = 6 for each; median = 10.5 and 55.4 nM, respectively). The boxes denote the interquartile range (IQR) (i.e., the 25th and 75th quartiles); the whiskers are 1.5´ IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. (B) Comparable distribution of reads for major genomic categories (i.e., mitochondria, rRNA, intergenic, intronic, and exonic; n = 6). The boxes denote the IQR (i.e., the 25th and 75th quartiles); the whiskers are 1.5´ IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. (C) Gene counts for transcripts per million (TPM) > 0.1 with SSsc PLUS compared with Nextera XT [n = 6; medians = 14,643 (SSlp) and 14,494 (Nextera XT)]. (D) Representative x-y plots of 2 example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate and the same cDNA library compared between Nextera XT and PLUS. R2 values are shown in the graph; Nextera XT R2 = 0.9961, PLUS R2 = 0.9978, and Nextera XT versus PLUS R2 = 0.9579.

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Benchmarking Single-Cell mRNA–Sequencing Technologies Uncovers Differences in Sensitivity and Reproducibility in Cell Types With Low RNA Content

    doi: 10.7171/3fc1f5fe.dbeabb2a

    Figure Lengend Snippet: Comparing library preparation of Nextera XT and SSsc PLUS. A) Higher library yields for SSsc PLUS (n = 6 for each; median = 10.5 and 55.4 nM, respectively). The boxes denote the interquartile range (IQR) (i.e., the 25th and 75th quartiles); the whiskers are 1.5´ IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. (B) Comparable distribution of reads for major genomic categories (i.e., mitochondria, rRNA, intergenic, intronic, and exonic; n = 6). The boxes denote the IQR (i.e., the 25th and 75th quartiles); the whiskers are 1.5´ IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. (C) Gene counts for transcripts per million (TPM) > 0.1 with SSsc PLUS compared with Nextera XT [n = 6; medians = 14,643 (SSlp) and 14,494 (Nextera XT)]. (D) Representative x-y plots of 2 example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate and the same cDNA library compared between Nextera XT and PLUS. R2 values are shown in the graph; Nextera XT R2 = 0.9961, PLUS R2 = 0.9978, and Nextera XT versus PLUS R2 = 0.9579.

    Article Snippet: Outliers are plotted as empty circles. ( C ) Gene counts for transcripts per million (TPM) > 0.1 with SSsc PLUS compared with Nextera XT [ n = 6; medians = 14,643 (SSlp) and 14,494 (Nextera XT)]. ( D ) Representative x - y plots of 2 example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate and the same cDNA library compared between Nextera XT and PLUS.

    Techniques: cDNA Library Assay